Then study more biologic actions of canstatin to the HUVEC-12 by Curve of growth assay and Flow cytometry. 通过绘制细胞生长曲线和流式细胞学技术进一步研究canstatin对血管内皮细胞的生物学作用。
Methods: 1. To determine the toxicity of alpha-terthienyl and deltamethrin to sensitive and resistant Aedes albopictus 4~ ( th) larvae by biologic assay. 用α-T和溴氰菊酯分别对白纹伊蚊幼虫敏感株及溴氢菊酯抗性株进行毒力测定。
The level of TNF-α in EAE model was detected by biologic assay and the level of IL-8 in EAE was examined by sandwich-enzymelinked immunosorbent assay ( ELISA). 同时采用生物活性测定法检测了EAE模型的TNF-α水平,采用双抗体夹心法酶联免疫吸附实验检测了EAE模型的IL-8水平。